医学论文-胃癌caspase-3表达及其与细胞凋亡的关系研究.doc

医学论文-胃癌caspase-3表达及其与细胞凋亡的关系研究
【摘要】  目的  研究caspase-3表达在胃癌发生发展中的作用。方法  采用免疫组化S-P法观察113例胃癌旁上皮细胞、原发灶癌细胞和浸润淋巴细胞以及转移灶癌细胞中caspase-3表达,TUNEL法检测上述组织的细胞凋亡,并分析原发灶癌细胞中caspase-3表达与胃癌临床病理特征的关系,并探讨caspase-3表达与细胞凋亡的关系。结果  caspase-%(57/113)的胃癌旁上皮细胞中表达,%(37/113)(P<);caspase-%(80/113),高于胃癌旁上皮细胞和原发灶癌细胞中阳性率(P<);%(25/43)的转移灶癌细胞中caspase-3表达阳性,其阳性率高于对应原发灶癌细胞(%,13/38)(P<);原发灶癌细胞中caspase-3表达与胃癌患者的年龄、性别以及胃癌肿瘤大小、浸润深度、转移、TNM分期、生长方式和分化程度无相关性(P>)。原发灶癌细胞和浸润淋巴细胞及转移灶癌细胞中细胞凋亡指数明显低于癌旁上皮细胞(P<)。结论  胃癌原发灶癌细胞中caspase-3表达下调和浸润淋巴细胞中caspase-3表达上调与胃癌发生有关,由caspase-3介导的细胞凋亡与胃癌的发生与发展有关。
【关键词】  胃癌  caspase-3  病理学  凋亡
【Abstract】 Purpose:To study the role of caspase-3 expression and apoptosis in carcinogenesis and progression of gastric cancer. Methods:Epithdial cells of mucosa adjacent to carcinoma, cancer ceils and infiltrating tymphocytes of primary foci and cancer cells of metastatic foci from 113 gastric cancers were studied using streptavidin-hiotin-immunoperoxidase immunohistochemistry with anti-caspase-3 antibody. Caspase-3 expression in cancer cells of primary foci pared with the clinicopathological features of gastric cancer. The apoptosis index (AI) of the abpve-mentioned specimens was studied by TUNEL method. Results:There was high immunohistoreactivity to caspase-3 in epithelial cells of mucosa adjacent to carcinoma from % (57/113) o