乌龙丹对慢性脑缺血大鼠松果体钟基因表达的影响.pdf

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560 南方医科大学学报(J South Med Univ) 2012;32(4)
基础研究
乌龙丹对慢性脑缺血大鼠松果体钟基因表达的影响
富祯祯 1,夏旸 2,彭康 1
南方医科大学 1中医药学院,2南方医院呼吸科,广东广州 510515
摘要:目的初步探讨慢性脑缺血对大鼠睡眠钟基因表达的影响及乌龙丹对其的调控作用。方法 24只雄性SD大鼠随机分为假
手术组、模型组、乌龙丹给药组。采用改良式双侧颈总动脉永久性结扎法建立大鼠慢性脑缺血模型,造模3周后,灌胃给药3周,
并持续监测各组大鼠体重,应用实时荧光定量PCR检测大鼠松果体钟基因Clock、Bmal1、Per1表达的变化。结果模型组较假
手术组 Clock mRNA、Per1mRNA 表达显著降低(P<, P<),Bmal1 基因表达无显著差异(P>);乌龙丹给药组的
Clock 基因水平高于模型组(P<),Bmal1基因表达较假手术组降低(P<),Per1无显著变化(P>)。结论慢性脑缺血
可能成为睡眠障碍的潜在病因,乌龙丹对慢性脑缺血引起的睡眠障碍有一定的治疗作用。
关键词:慢性脑缺血;钟基因;Clock;Bmal1;Per1;乌龙丹;睡眠障碍
中图分类号: 文献标志码:A 文章编号:1673-4254(2012)04-0560-05
doi: .1673- s/detail/
Effects of Wulongdan on expression of pineal clock genes in rats with chronic cerebral
ischemia
FU Zhenzhen1, XIA Yang2, PENG Kang1
College of Traditional Chinese Medicine1, Department of Respiratory Diseases, Nanfang Hospital2, Southern Medical University,
Guangzhou 510515, China
Abstract: Objective To explore the changes in the expression of pineal clock genes in rats with chronic cerebral ischemia and
evaluate the effect of intervention with Wulongdan, a traditional Chinese medicinal preparation, on these changes. Methods
Male SD rats were randomly divided into sham-operated group, chronic cerebral ischemia model group, and treatment group.
In the latter two groups, chronic cer