高效液相色谱测定米糠中水溶性维生素.doc

高效液相色谱法测定米糠中的水溶性维生素
(江南大学化工与材料工程学院,江苏无锡214122)
摘要:高效液相色谱法同时测定米糠中四种水溶性维生素VB1,VB6,烟酸,烟酰***的含量。在Hypersil C18 (250 cm× mm ., μm)上,以体积比为88:%磷酸水溶液-甲醇为流动相, mL/min流速等度洗脱,在266nm波长下检测。各组分在8min能基线分离。×10-6g/mL~×10-5g/mL范围内呈线性关系,VB1、VB6、烟酸和烟酰***×10-6 g/mL、×10-6g/mL、×10-6 g/mL、×10-6 g/mL。经简单预处理,可以对米糠和淘米水中的这四种维生素的含量进行测定,%~%之间。该法简便、快速、准确、灵敏,对复杂体系水溶性维生素的测定具有重要的参考价值。
关键词:水溶性维生素;米糠;高效液相色谱
Determination of four water-soluble vitamins in rice bran by reverse-phase high performance liquid chromatography
CAO Rui, CAO Yu-hua, LI Nan, GONG Wen-jun, KONG Ling-yao, WANG Yun
School of Chemical and Material Engineering, Southern Yangtze University, Wuxi 214122
Abstract: High performance liquid chromatography (HPLC) has been applied for the simultaneous determination of four kinds of water-soluble vitamins including vitamin B1, vitamin B6, nicotinic acid, and nicotinamide in rice bran. The separation was performed on a Hypersil C18 column (250 cm× ., μm) with a mobile phase of % H3PO4- methanol (88:12, V/V) at flow rate of mL /min, and detection wavelength was 266 nm. Four vitamins had good linearity in the range of ×10-7g/mL - ×10-5g/mL, with detection limit of ×10-7 g/mL, ×10-6g/mL, ×10-7g/mL and ×10-7 mg/L for vitamin B1, vitamin B6, nicotinic acid and nicotinamide, respectively. Baseline separat