共刺激分子B7-H1对人肝癌细胞生物学特性的影响.doc


共刺激分子 B7-H1 对人肝癌细胞生物学特
性的影响#
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摘要:目的:探讨 RNA 干扰技术沉默 B7-H1 基因对肝癌细胞 HCCLM3 生长侵袭及体内成
瘤能力变化的影响。方法:通过慢病毒载体将 B7-H1 小干扰 RNA 片段稳定转染入 HCCLM3
细胞。流式细胞仪检测 sh-RNA-B7-H1 组(感染 B7-H1 慢病毒载体组)、shRNA 对照组(感染
阴性对照慢病毒载体组)K-8 法检测转染后 HCCLM3 细胞生长情况;
Transwell 实验检测转染后 HCCLM3 细胞运动侵袭能力;裸鼠皮下接种检测转染后 HCCLM3
细胞体内成瘤及移植瘤生长能力。结果:慢病毒载体干扰 RNA 可以明显降低 sh-RNA-B7-H1
组 HCCLM3 细胞的体外侵袭和运动能力(P<),K8 法检测细胞周期
及体外生长能力发现各组相比差异无显著性(P>)。裸鼠体内实验发现 sh-RNA-B7-H1 组
HCCLM3 细胞的成瘤能力及移植瘤的生长能力显著低于对照组(P<)。结论:本研究通
过 RNA 干扰技术证实下调 B7-H1 表达可直接抑制肝癌细胞的体内生长及体外运动侵袭能
力。
关键词:肝细胞癌;复发转移;B7-H1;RNA 干扰。
中图分类号:
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Biological effects of costimulatory molecule B7-H1 on
human hepatocellular carcinoma
GAO Qiang, WANG Xiaoying
(Liver Cancer Institute and Zhongshan Hospital, Fudan University, Shanghai, 200032)
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Abstract: Objective To investigate the effects of B7-H1 expression on the in vitro proliferatin and
invasion as well as in vivo tumor growth of human hepatocellular carcinoma (HCC) cell
HCCLM3. Method Lentivirus mediated RNA interference was used to knock down B7-H1
expression in human HCC cell line HCCLM3. The cell cycle distribution of B7-H1 knockdown
HCCLM3 cells (sh-RNA-B7-H1) and control HCCLM3 cells (sh-RNA-Ctrl) was analyzed using
flow cytometry. In vitro cell proliferation and migration/invasion was detected K-8 assay
and Tanswell assay respectively. Subcutaneous nude m