木瓜蛋白酶水解海洋鱼鳞蛋白的工艺研究.pdf

现代食品科技 Modern Food Science and Technology 2011, ,

木瓜蛋白酶水解海洋鱼鳞蛋白的工艺研究

杨丽虹 1,吴琴琴 1,唐旭 2,徐长安 2,何建林 2
(,福建厦门 361005)(,福建厦门 361005)
4
摘要:研究了木瓜蛋白酶水解鱼鳞蛋白的单一影响因素,并采用 L9(3 )正交实验,考察了温度、时间、酶量、底物浓度等因素
对水解鱼鳞蛋白的影响。确定了木瓜蛋白酶水解鱼鳞蛋白工艺条件,即温度 65 ℃,时间 75 min,酶量 g/g 鱼鳞,底物浓度 10%,
适合规模化生产。
关键词:木瓜蛋白酶;海鱼鳞蛋白;水解
文章篇号:1673-9078(2011)12-1484-1486
Hydrolysis Process of Sea-fish Scale Protein Catalyzed by Papain
YANG Li-hong1, WU Qin-qin1, TANG Xu2, XU Chang-an2, HE Jian-lin2
( of Oceanography, Xiamen University, Xiamen 361005, China)
( Institute of Oceanography, SOA, Xiamen 361005, China)
Abstract: Hydrolysis of the protein from sea-fish scales by papain was studied using orthogonal experiment method. Effects of several
factors on hydrolysis were investigated. Based on the research work, the optimum treatment condition was summarized as follows: temperature
65 ℃, hydrolysis time 75 minutes, enzyme g/g scale and substrate concentration 10%. The result indicated that the conditions were
suitable for large-scale production.
Key words: papain; sea-fish scales protein; hydrolysis

改革开放以来,海洋水产品加工方面取得了长足美国红鱼鱼鳞:购自福建东山,经洗涤、脱脂和
的进步。也产生了占 30%的废弃物,其中有 10%的左脱钙处理后干燥备用;木瓜蛋白酶:南宁庞博生物工
右鱼鳞资源未得到很好利用[1]。鱼鳞中含有丰富的胶程有限公司,酶活力 80 万 U/g。
原蛋白,是动物体内含量最丰富的蛋白质[2]。具有促电子天平:METTLER TOLEDO AL104 型;烘箱:
进伤口癒合和组织修复的重要功能[3]。同