前列腺癌组织中PTEN蛋白的表达及其与肿瘤细胞凋亡的关系.doc

前列腺癌组织中PTEN蛋白的表达及其与肿瘤细胞凋亡的关系
【关键词】前列腺肿瘤
【Abstract】 AIM: To investigate the expression of PTEN protein in tissues of prostatic carcinoma (Pca) and its correlation with the apoptosis of cancer cells. METHODS: Expression of PTEN protein and apoptotic index (AI) were detected by SP immunohistochemical and terminal deoxynucleotidyl transferasemediated dUTP biotin nick end labeling (TUNEL) technique in 42 cases of Pca and 10 cases of normal prostate (NP). The relationship between the PTEN gene deletion and AI in Pca was analyzed. RESULTS: All of the ten cases of NP were positive for PTEN protein. The overall deletion rate of PTEN gene in Pca was % and it was strongly associated with the degree of differentiation, clinical stage and lymph metastasis in Pca (P<), but it had no correlation with the age of patients. The PTEN gene deletion was negatively correlated with AI in Pca (r = -, P<). CONCLUSION: There is a higher deletion rate of PTEN gene in Pca, which may play an important role in the genesis and development of some Pca and result in the reduction of cell apoptosis in Pca. Detection of PTEN protein and AI in Pca may be helpful to evaluate the degree of cell differentiation, decide on therapeutic strategies and deduce progn
osis.
【Keywords】 PTEN protein; prostatic neoplasms; immunohistochemistry
【摘要】目的: 探讨抑癌基因PTEN蛋白在前列腺癌(Pca)组织中的表达及其与肿瘤细胞凋亡的关系.