慢性压力负荷性心衰大鼠肾脏细胞凋亡.doc

慢性压力负荷性心衰大鼠肾脏细胞凋亡
作者:周泉李志梁胡高频王素华陆青
【关键词】心力心衰
关键词: 大鼠;心力心衰;缺口末端标记术;肾脏细胞;凋亡
摘要:目的了解慢性心脏后负荷增加导致慢性心力衰竭时肾脏细胞凋亡的情况,探讨慢性心脏后负荷增加及引起慢性心力衰竭时影响肾脏功能不全的机制. 方法复制慢性后负荷增加引起心力衰竭大鼠(n=24)模型,检测血液动力学(BP,LVSP)变化,应用末端转移酶介导的缺口末端标记法(TUNEL)检测肾脏细胞凋亡. 结果对照组大鼠肾脏及慢性后负荷增加早期肾脏均未出现细胞凋亡,心力衰竭时肾脏出现细胞凋亡,细胞凋亡程度肾髓质(36-ND)>肾近曲小管(17-ND)>肾小球(6-ND),肾血管平滑肌细胞及致密斑亦出现细胞凋亡. 结论慢性后负荷增加引起心力衰竭时肾脏出现细胞凋亡,它与慢性心力衰竭密切相关,是肾功能不全的重要原因.

Keywords:rat;heart failure;TUNEL;renal cells;apoptosis

Abstract:AIM To observe renal cells apoptosis in chronic heart failure which resulted from the chronic excessive heart post- study the molecular mechanism of renal failure in chronic heart failure resulting from the excessive post- The models of chronic heart failure which resulted from chronic excessive post-load were made in rats(n=24).The change of blood dynamics was presence of apoptotic renal cells was demonstrated by the method of terminal deoxynucleotidyl transferas
e mediated dUTP nick end labeling(TUNEL).RESULTS The pres-ence of apoptotic renal cells was not demonstrated in the con-trol groups and the early stage of chronic excessive post-load,and was demonstrated in chronic heart failure degree of renal medulla’s apoptosis(36-ND)was degree of renal juxtaglomerular tubule’s apoptosis(17-ND)was lower;the degree of glomerulu’s(6-ND)was low- apoptotic renal