732型阳离子交换树脂分离猫豆中的猫豆胍.doc

732型阳离子交换树脂分离猫豆中的猫豆胍
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作者:黄增琼,蒋伟哲,黄兴振,陆璐,巫世红
【摘要】目的考察732型阳离子交换树脂分离猫豆中猫豆胍(MDG)的工艺参数。方法以猫豆胍洗脱率为考察指标,运用正交实验法考察了洗脱液浓度、用量和洗脱速度对洗脱效果的影响。结果在考察范围内,最佳分离条件为:150倍量1%氨水, ml/min。在优化条件下,%。结论该工艺方法简单,成本低廉,适于工业大生产。
【关键词】猫豆; 猫豆胍; 离子交换; 阳离子树脂; 分离纯化
Abstract:ObjectiveTo study the process parameters of MDG separated from the seed of Stizolobium cochinchinensis by 732 cation exchange orthogonal test was adopted to study the eluting effect of MDG with the elution rate as an index, and concentration of eluant, amount, elution velocity as the influence factors. ResultsThe results showed that the best separating conditions were 150 times of 1% ammonia hydrate as eluant, and the elution velocity was . Under the optimal conditions, the elution rate was %. ConclusionThe separated procedure is simple, the cost is low,and it is suited for industial produce.
Key words:The seed of Stizolobium cochinchinensis; MDG; Ion exchange; Cationic resin; Separation and purification
猫豆又名猫爪豆、狗爪豆、白藜豆、龙爪豆,是豆科植物龙爪藜豆Stizotobium cochinchinensis (Lour). Tang et Wang的种子[1],广西是最主要的产区。猫豆中主要成分为左旋多巴,是国内提取左旋多巴最主要的原料药材。笔者采用制备液相色谱层析系统AKTA Explorer 100从猫豆中提取分离得到一种单体成分,经化学结构鉴定,该成分为首次从猫豆中分离获得,暂命名为猫豆胍[2]。药理学研究表明,该化合物具有一定的镇静催眠作用。为了更好地利用猫豆中的猫豆胍,提高猫豆的综合利用率,改变猫豆单一提取左旋多巴的生产模式,探寻适合工业化生产猫豆胍的工艺,本文对732型阳离子交换树脂分离猫豆胍的工艺条件进行了探索和优化。
1 仪器与材料
LC-9000