TPPA和RPR联合检测梅毒抗体的实验研究.doc

TPPA和RPR联合检测梅毒抗体的实验研究
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作者:王育瑛,赵文波,彭敏峰,王莉
【摘要】:[目的]分别用TPPA和RPR两种方法检测梅毒抗体,比较两种方法在梅毒诊断及疗效观察方面的意义。[方法]对疑为梅毒患者血清标本共80份分别进行TPPA和RPR检测。[结果]63份梅毒患者TPPA阳性,%,灵敏度100%;RPR39例阳性,%,%。[结论]TPPA的灵敏度和特异性均优于RPR,故TPPA法检测梅毒特异性抗体,应作为临床梅毒确诊的主要检测手段,RPR法检测梅毒非特异性抗体,应作为临床梅毒的筛差实验以及梅毒治疗的监测手段。
【关键词】梅毒;TPPA; RPR
Abstract:[Objective] Compare the significance on syphilis diagnosis and curative effect by detection of syphilis antibodies using two methods, TPPA and RPR, respectively.[Methods] Using TPPA and RPR ways to detect 80 serum samples of suspected syphilis patients. [Results]63 samples were positive in syphilis test using TPPA. Positive rate was %, sensitivity 100%; 39 samples were positive in syphilis test using TPPA. Positive rate was %, sensitivity %.[Conclusions]The TPPA is superior than RPR in sensitivity and specificity. Therefore, detecting specific syphilis antibody by TPPA can be used as the main method in clinical diagnosis of syphilis, while detecting nonspecific syphilis antibody by RPP can be used as the screening test of syphilis patients and the monitoring method to cure syphilis.
Key words: syphilis; TPPA; RPR
梅毒是由梅毒螺旋体引起的一种慢性系统性传播疾病,人体感染后,体内能产生两种抗体:一类是针对梅毒螺旋体产生的特异性抗体,即梅毒螺旋体抗体(IgG和IgM);另一类是非特异性类脂质抗体,即反应素。梅毒的临床表现非常复杂,临床上易被漏诊或误诊为“药诊,银屑病,斑秃”等皮肤病[1]。因此,梅毒的检查结果对病情的判断就显得极为重要。目前梅毒检测应用最普遍的实验室检测方法是梅毒血清