白介素8对兔腹主动脉粥样硬化狭窄的影响.doc

白介素8对兔腹主动脉粥样硬化狭窄的影响
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作者:齐晓勇孟存良李英肖党懿刘惠良
王天红袁华兵倪艳辉刘光武振朝郜利会
【摘要】目的观察白介素8(IL8)水平对兔腹主动脉粥样硬化狭窄的影响。方法新西兰大白兔36只随机分为实验组、IL8单克隆抗体治疗组、对照组。实验组和治疗组动物通过穿刺股动脉送入球囊拉伤腹主动脉。治疗组动物通过耳缘静脉注射IL8单克隆抗体进行干预。对照组动物只进行股动脉穿刺和留置鞘管,不进行球囊扩张拉伤。分别于实验前,实验后4 h、1 d、3 d,1 w、2 w、4 w,耳缘静脉采血4 ml,检测三组动物血清IL8水平,4 w后处死动物进行病理组织学检查,应用光学显微镜和计算机病理图像分析系统分析测定以下指标:内膜中膜厚度、面积,计算血管狭窄程度。结果实验组动物球囊损伤4 h后IL8水平即开始升高,术后1 d达到峰值,持续增高4 w,且明显高于治疗组和对照组。治疗组和对照组动物IL8水平无上述变化规律。实验组动物腹主动脉明显狭窄,组织学检查内膜面积和中膜面积、血管狭窄程度等指标大于治疗组和对照组(),治疗组管腔狭窄,对照组动物管腔无明显变化,治疗组与对照组比较无显著性差异()。相关分析显示,IL
8水平与内膜厚度、内膜面积、中膜厚度、中膜面积等呈正相关(,,,,)。结论 IL8水平高表达引起腹主动脉粥样硬化狭窄的产生,经IL8单克隆抗体治疗后动脉狭窄程度明显减轻。IL8水平与动脉狭窄程度呈正相关,是发生腹主动脉粥样硬化狭窄的独立危险因素。
【关键词】腹主动脉;动脉粥样硬化;炎性反应;白介素8;单克隆抗体
【Abstract】 Objective To observe the effects of interleukin8 (IL8) on atherosclerosis(AS) of abdominal aorta in rabbits. Methods Thirtysix New Zealand white rabbits were randomly divided into experiment, treatment and control groups, each group 12 cases. Rabbits in experiment and treatment groups were injuried in abdominal aorta by balloon inflation after punctured in femoral artery. Monoclonal antibody of IL8 was injected by venous in rabbits of treatment group. Rabbits in control group were punctured only in femoral artery. Peripheral blood was collected before experiment and 4 h, 1, 3 d, 1, 2, 4 w later. The level of IL8 was measured by enzyme linked immunosorbent assay (ELISA) respectively. The luminals of abdo
mial aorta in three groups were observed 4 w later. Histopathologic examination was performed. Such contents as follows were assayed by light microscope puter image analysis system: luminal area, intima and tunica media area, angiostenosis of blood vessel. Results The level of IL8 was raised in 4 h and achieved to peak in 1 d after balloon inflation in rabbits of experiment group. The higher level of this inflammatory factor lasted 4 w. There was no variation above in treatment and c