前列腺酸性磷酸酶在非前列腺腺癌中的表达.doc

前列腺酸性磷酸酶在非前列腺腺癌中的表达
作者:王毅,王芳,宿晓云,原田守,山田亮,伊东恭悟,颜炜群
【摘要】目的: 探讨前列腺相关抗原前列腺酸性磷酸酶(PAP)在非前列腺癌中的表达水平. 方法: 分别用RTPCR方法和Western Blot方法检测多种腺癌细胞系中PAP mRNA和PAP蛋白的表达水平;用免疫组织化学方法检测多种腺癌肿瘤组织中的PAP蛋白的表达水平. 结果: 3种结肠癌细胞(colo 201, colo 205和colo 320)、3种胃癌细胞(MKN7, MKN28和 MKN45)和7种乳腺癌细胞(OCUBF, OCUBM, R27, MCF7, CRL1500, YMB1E和MDAMB231)表达PAP mRNA和PAP蛋白,结肠癌、胃癌和乳腺癌肿瘤组织中PAP呈阳性表达. 在检测的5种肾细胞癌细胞和肾细胞癌组织中均未发现有PAP的表达. 结论: PAP是结肠癌、胃癌和乳腺癌的新靶点.
【关键词】酸性磷酸酶;前列腺;腺癌
【Abstract】 AIM: To study the expression of prostatic acid phosphatase (PAP) ,a prostaterelated antigen, in nonprostate cancer cells or tissues. METHODS: A variety of adenocarcinoma cell lines were examined for their PAP expression at the mRNA and protein levels by RTPCR and Western Blot analysis, respectively. The PAP expression in cancer tissues was also examined by immunohistochemical staining. RESULTS: Three kinds of colon cancer cells(colo 201, colo 205 and colo 320), 3 kinds of gastric cancer cells (MKN
7, MKN28 and MKN45), and 7 kinds of breast cancer cell lines (OCUBF, OCUBM,R27, MCF7, CRL1500, YMB1E and MDAMB231) as well as cancer tissues were found to be positive for PAP at both the mRNA and protein levels. But is was not found in 5 kinds of kidney cancer cells or tissues. CONCLUSION: PAP is a new target of nonprostate adenocarcinomas, including colon, breast and gastric cancers.
【Keywords】 acid phosphatase