PBS缓冲液的配制.doc

PBS是最普遍不过的实验室缓冲液,但其配方各异。以下是总结: 母液的配制: :-12H2O,:-2H2O,溶于1000ml水各种浓度PB(pH=)的配制:(pH=,100ml):,,即可。(pH=)按相应比例适当稀释即可,如:(PH=):,加水稀释至1000ml即可。(PH=):,加水稀释至1000ml即可。(PH=):,加水稀释至1000ml即可。 若需要NaCl的话,%(g/100ml)即可。 另:(100ml)配方:pH    (ml)      (ml)                                  92                    8                90                                                      85                                                                                                                                                                                        51                            45                            38                            33                          28                            23                            19ml                          16                            13                                                                                7                                              (PBS)配制方法称取8gNaCl、、,溶于