鬼臼毒素固体脂质纳米粒经皮渗透和皮肤贮留研究.doc

鬼臼毒素固体脂质纳米粒经皮渗透和皮肤贮留研究
(作者:___________单位: ___________邮编: ___________)
【摘要】目的研究鬼臼毒素固体脂质纳米粒经皮渗透和皮肤贮留情况。方法采用Franz扩散池测定鬼臼毒素固体脂质纳米粒的经皮渗透速率,扩散池与供给池之间为大鼠或家兔腹部皮肤, cm2。在体外透皮试验完结后,取下皮肤,剪碎匀浆,用50%异丙醇生理盐水提取,提取液处理后用HPLC测定药物浓度。%,% (n=5),粒径在50~200 nm之间。固体脂质纳米粒作为载体时,鬼臼毒素经大鼠和家兔离体腹部皮肤的平均渗透速率和累积透过量较市售酊剂明显降低,同时药物贮留量较市售酊剂明显提高。结论鬼臼毒素固体脂质纳米粒具有较强的皮肤靶向性。
【关键词】鬼臼毒素;固体脂质纳米粒;经皮渗透;皮肤贮留
Abstract:Objective To study in vitro transdermal ration and retention of podophyllotoxin solid lipid nanoparticles(SLN). Methods The in vitro transdermal ration of podophyllotoxin from SLN was determined by Franz diffusion cells. Between partment and partment was abdominal skin of rats or rabbits,the avaiable diffusion area of cells was cm2. The sample was analyzed by HPLC after treatment. After in vitro transdermal ration pleted,the skin were taken down from cells,cut and homogenated,then extracted with 50% isopropanol
normal saline. The extracting solution was analyzed by HPLC. Results The encapsulation efficiency of obtained podophyllotoxin solid lipid nanoparticles were % on average,with RSD was % (n=5) and diameter between 50~200 nm. When solid lipid nanoparticles as deliverer,the average transdermal rate and cumulative transdermal quantity of podophyllotoxin through rat and rabbit abdominal skin in vitro were significantly lower than marketed tincture,wh