2021年高效液相色谱法测定人血浆中全反式维甲酸血药浓度.docx

高效液相色谱法测定人血浆中全反式维甲酸血药浓度

　　关键词 血药浓度
摘要:目标 建立高效液相色谱法测定人血浆中全反式维甲酸浓度。方法 色谱柱为Supelcosil LC18DB柱（5 μm, mm×250 mm）；流动相为甲醇水冰乙酸(体积比82∶17∶1)；柱温为室温； mL・min1；检测波长为340 nm。结果 全反式维甲酸血药质量浓度在10～1000 ng・mL1范围内，和峰面积比有良好的线性关系（r=）， ng・mL1。平均方法回收率为（±）%（n=15）。日内RSD≤%，日间RSD≤%。结论 本方法简单、快速、灵敏、重现性好，适合用于全反式维甲酸临床血药浓度监测及人体药代动力学研究。
　　关键词:全反式维甲酸；血药浓度；高效液相色谱法
　　Abstract: Objective To establish a HPLC method for determination of alltrans retinoic acid in human plasma. Methods A Supelcosil LC18DB column (5 μm, mm×250 mm) was adopted. Mobile phase was a mixture of methanol,water and glacial acetic acid（82∶17∶1）. The flow rate was mL・min1. The UV detection wave was 340 nm. ResultsThe assay procedure was shown to produce linear calibration curves over the range of 10 ng・mL1 to 1000 ng・mL1 of alltrans retinoic acid in plasma (r=). Within the range,the recovery rate was (±)%,and the intraday RSD and interday was less than % and %,respectively. The detection limit of all trans retinoic acid was ng・mL1. Conclusions This method is repeatable,convenient and sensitive for plasma concentration monitoring and clinical pharmacokinetics study of alltrans retinoic acid.
　　Key words:alltrans retinoic acid; plasma concentration; HPLC
全反式维甲酸(alltrans retinoic acid,ATRA) 是维生素A 的天然氧化代谢产物，可抑制白血病细胞的增殖，诱导白血病细胞分化成熟，是现在诊疗急性早幼粒细胞白血病(APL)的有效药品。ATRA对APL初发患