Nuclear ic Resonance (Nmr) Spectroscopy Of Proteins (2).pdf

Nuclear ic Secondary article
Resonance Spectroscopy of Article Contents
. Introduction
Proteins . NMR Structure Determination of Proteins
. New Experimental Approaches for High-molecular
Mass Systems
Kurt Wu¨thrich, Swiss Federal Institute of Technology (ETH), Zu¨rich, Switzerland
. putational Techniques in NMR Structure
Determination
Nuclear ic resonance (NMR) spectroscopy enables the determination of three- . Chemical Shifts and Protein Structure
dimensional protein structures at atomic resolution under near-physiological conditions in . NMR of ic Proteins
solution. In structural biology, plements X-ray crystallography, which provides
similar information on proteins in single crystals.
Introduction
For the NMR sample preparation, the protein is
2 1
For about 25 years, X-ray diffraction in single crystals was dissolved at about 1 mmol L concentration in mL
unique in its ability to determine three-dimensional (3D) of water. The ionic strength, pH, temperature, and possibly
structures of proteins at atomic resolution. The situation the concentration of additives may then be adjusted, for
changed in 1984 with pletion of a protein structure example to ensure near-physiological conditions or dena-
determination by nuclear ic resonance (NMR) turing conditions, and so on.
spectroscopy in solution, and today NMR is a second, The demands on NMR experiment