肺癌患者血浆游离性肿瘤dna研究.pdf

? 601? 肿瘤防治研究 2003 年第 30 卷第 2 期
肺癌患者血浆游离性肿瘤 DNA 的研究
1 2 2 3
易 　斌 ,杨和平 ,阮志华 ,钱桂生
Astud yon plasmaDNAfrom patientswithlun gcancer
YIBin,YANGHe 2pin g,RUANZHI 2hua,etal
Department of Anesthsia , the South 2West Hospital Af filiated to The Third Militar y Medical Universit y ,
Chongqin g 400038 , China
Abstract :Objective Tostud ythedia gnosticvalueofLOHandMSIof3 pmicrosatellite pointinfree plasma
DNAoflun gcancer patients. Methods Weusetechni quesofPCRandsliver 2dyetodetectLOHandMSIofthree
microsatellitesitesof plasmaDNAonchromosome3 pfrom37sur geryfreshtissuesandwholebloodsam plefrom
94 patients. Results The plasmafreeDNAconcentrationofthemostlun gcancer patientswasabovemicro 2
%oftissuesand plasmaswere gnificantdifferencewasfoundondifferent
stagesand pathologyt patientswithl ymphnodemetastasiswassi gnificantdifferentwiththe
patientswithoutl ymphnodemetastasis. Conclusion 　PlasmaDNAoflun gcancerisa goodmediumof genede2
tectionandwillbeusedwides preadinthefuture.
Keywords :Lun gcancer;PlasmaDNA;Microsatellite;Genedia gnosis
摘 　要 :目的 　研究肺癌患者血浆游离 DNA 微卫星不稳定和杂合性缺失在肺癌诊断中的价值