难免流产蜕膜组织遗传印记基因PEG10的表达.pdf

HEREDITAS (Beijing) 2008 年 6 月, 30(6): 735―740
ISSN 0253-9772 告
DOI: .
难免流产蜕膜组织遗传印记基因 PEG10 的表达
梁小妍, 刘学庆, 丁裕斌, 陈雪梅, 王应雄, 何俊琳
重庆医科大学公共卫生学院生殖生物学研究室, 遗传优生教研室, 重庆 400016
摘要: 采用半定量逆转录聚合酶反应(RT-PCR)、原位杂交、免疫印记(Western blot)及免疫组织化学技术检测了
36 例难免流产患者蜕膜组织 PEG10 (Paternally expressed gene 10) mRNA 及蛋白的表达与分布, 并以 36 例同期
正常早孕妇女为对照, 研究遗传印记基因在难免流产蜕膜组织中的表达, 探讨其在自然流产中的作用。
RT-PCR 结果显示, PEG10 在两组蜕膜组织中均有表达, 正常妊娠组平均表达水平为 ±, 难免流产
组为 ±, 两组比较具有显著性差异(P<)。原位杂交、免疫组化及 Western blot 分析也显示 PEG10
的表达规律与 RT-PCR 结果相吻合。研究结果表明, 遗传印记基因 PEG10 维持一定水平的表达对早期胚胎发
育和正常妊娠的维持有重要意义, 而其表达下调可能是导致难免流产的原因之一。
关键词: 遗传印记; PEG10; 蜕膜; 难免流产
ic imprinted gene PEG10 expression in deciduas from
inevitable abortion
LIANG Xiao-Yan, LIU Xue-Qing, DING Yu-Bin, CHEN Xue-Mei, WANG Ying-Xiong,
HE Jun-Lin
Laboratory of Reproductive Biology, Department of ics, School of Public Health, Chongqing Medical University, Chongqing 400016,
China
Abstract: The expression and distribution of the imprinted paternally expressed gene 10 (PEG10) in decidual tissues of 36
patients with inevitable abortion and 36 normal pregnant (NP) women were examined by RT-PCR, immunohistochemistry,
in situ hybridization and Western blot. Decidual PEG10 mRNA expression was significantly lower in the patients than that
in the NP group by RT-PCR (P<). The characteristic PEG10 expression was