透明颤菌血红蛋白基因在产黄青霉中.docx

密级:
分类号:
学校代码：10075
学 号：022996
理学硕士学位论文
透明颤菌血红蛋白基因在产黄青霉中
的克隆与表达
学位申请人： 李兵
指导教师：
朱宝成教授
赵晓瑜教授
学位类别：
constructed on the recombined plasmid pUC18-vgb, and both plasmids were transformed into . The pUC18-vgb> and pMIQ-vg" expressed Vitreoscilla hemoglobin(VHb) in ,with the inspections of the protein electrophoresis and CO difference constructed shuttle plasmid pMIGrug" with chloramphenicol-resistance was transformed into penicillium chrysogenum protoplast by the use of electroshock. Some chloramphenicol -resistance fungus were filtrated on the chloramphenicol culture medium (chloramphenicol thickness 50mg/ml).The vgb gene was successfully recombined into the penicillium chrysogenum genome through the PCR inspection, and the transformed successfully expressed the Vitreoscilla hemoglobin protein in low-oxygen ferment. The transformed penicillium chrysogenum was more ascendant than the original fungus in hight-oxygen ferment on output of the aimed production. Forther more mycelium growth quantity and the fermentation value enhanced % and %.
The vgb was expanded in application area through the study, and the research can also afford the foundation for vgb research in other filaceous fungus. The results are also beneficial to reconstruct other antibiotic-production fungus.
key words: penicillium chrysogenum'. protoplast; Vitreoscilla hemoglobin protein; transformation; Fermentation value
目录
第1章文献综述 1
1
1
1
2
3
（vg。）及其研究进展 4
4
VHb 的结构 4
vgb基因组成及特点 5
6
vgb基因表达的调控 7
8
9
第2章材料与方法 10
10
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11
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PCR 扩增目的片断 14
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