1206例呼吸道感染儿童肺炎支原体DNA.doc

1206例呼吸道感染儿童肺炎支原体DNA
钟伟明,郭静,陈俏[摘要]目的:了解儿童肺炎支原体(MP)感染情况。方法:采用荧光定量PCR(FQ-PCR)技术检测1206例疑似MP感染患儿的咽拭子标本中的MP-DNA。结果:1206例呼吸道感1206例呼吸道感染儿童肺炎支原体DNA
钟伟明,郭静,陈俏[摘要]目的:了解儿童肺炎支原体(MP)感染情况。方法:采用荧光定量PCR(FQ-PCR)技术检测1206例疑似MP感染患儿的咽拭子标本中的MP-DNA。结果:1206例呼吸道感染患儿中MP-DNA阳性435例,%。结论:提示FQ-PCR可快速、敏感、准确地定量检测标本中MP-DNA,了解MP在患儿体内感染情况,有助于临床明确诊断与治疗方案的选择。
[关键词]肺炎支原体;荧光定量聚合酶链反应;呼吸道感染
[中图分类号];[文献标识码]B[文章编号]1673-7210(2010)10(b)-060-02
TheanalysisoftheresultsofdetectionmycoplasmapneumoniaeDNAof1206casesofrespiratoryinfectionsinchildren
ZHONGWeiming,GUOJing,CHENQiao
(DepartmentofLaboratory,MaternalandChildHealthHospitalofGuigangCity,Guigang537100,China)
[Abstract]Objective:Toexploretheinfectionofmycoplasmapneumoniae(MP):1206throatswabsamplesofsuspectedMPinfectionMP-DNAweredetectedbyfluorescencequantitativepolymerasechainreaction(FQ-PCR).Results:In1206samplesofrespiratoryinfectioninchildren,theMP-DNAcopieswas435cases,%.Conclusion:FQ-PCRmethodforMP-DNAquantitativedetectionissimple,sensitive,,whichiscontributetotheclinicaldiagnosisandtreatmentoptionsofrespiratoryinfection.
[Keywords]Mycoplasmapneumoniae;Fluorescencequantitativepolymerasechainreaction;Respiratoryinfection
肺炎支原体(mycoplasmapneumoniae,MP)是小儿呼吸道感染的重要病原体之一,除呼吸道感染(咽炎、支气管炎、肺炎)外,MP尚可通过血行播散或免疫机制引起脑炎、肾炎、心肌炎等多种肺外并发症[1-2],对儿童健康有较大危害性。近年来MP感染的发病率