Assisted reproductive technologies impair the expression and methylation of insulin-induced gene 1 and sterol regulatory element-binding factor 1 in the fetus and placenta.pdf.pdf

Assisted reproductive technologies
impair the expression and
methylation of insulin-induced gene 1
and sterol regulatory element-binding
factor 1 in the fetus and placenta
Hangying Lou, .,a,b Fang Le, .,a Yingming Zheng, .,a Lejun Li, .,a Liya Wang, .,a
Ning Wang, .,a Yimin Zhu, .,a,b Hefeng Huang, .,a,b and Fan Jin, ,b
a Center of Reproductive Medicine, Women's Hospital, School of Medicine, Zhejiang University, and b Key Laboratory of
Reproductive ics, Ministry of Education, Hangzhou, People's Republic of China
Objective: To evaluate the cholesterol metabolism linked to assisted reproductive technology (ART) by analyzing the expression levels
and DNA methylation patterns of the insulin-induced gene (INSIG), sterol regulatory element-binding protein (SREBP), and SREBP
cleavage-activating protein in the fetus and placenta.
Design: Experimental research study.
Setting: An IVF center, university-affiliated teaching hospital.
Patient(s): Four patients groups were recruited: pregnancies after IVF/intracytoplasmic sperm injection (ICSI) (n ¼ 55), natural pregnan-
cies (n ¼ 40), multifetal reduction after IVF/ICSI (n ¼ 56), and multifetal reduction after controlled ovarian hyperstimulation (COH) (n ¼ 42).
Intervention(s): Expression and DNA methylation of INSIG-SREBP- SREBP cleavage-activating protein in the fetus and placenta
samples were