IL1β511位点和TNFα308位点基因多态性与陕西汉族非贲门胃癌的关系.doc

IL1β511位点和TNFα308位点基因多态性与陕西汉族非贲门胃癌的关系
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作者:贾皑,龚均,厉英超,苌新明,郝志明,董蕾
【摘要】目的探讨促炎症因子白细胞介素(IL)1β基因启动子区511位点及肿瘤坏死因子α(TNFα)308位点基因多态性与陕西汉族非贲门胃癌易感性的关系。方法用聚合酶链反应和限制性片段长度多态性(PCRRFLP)的方法对陕西汉族106例非贲门胃癌及108例非溃疡性消化不良患者(对照组)进行IL1β启动子的511位点和TNFα的308 位点多态性分析。结果 IL1β基因启动子、CT、%、%、33%%、%、%,无显著性差异()。等位基因C、%、%%、%,其基因型频率和等位基因频率在胃癌组和对照组比较均无显著性差异()。TNFα的308位点的基因型GG、GA、%、%、%%、%、%,等位基因G、%、%%、%,其基因型频率和等位基因频率在胃癌组和对照组比较均无显著性差异()。结论 IL
1β启动子511位点基因多态性及TNFα308基因多态性与陕西汉族非贲门胃癌之间未发现相关关系。
【关键词】白细胞介素1;肿瘤坏死因子α;基因多态性;胃癌;汉族
ABSTRACT: Objective To investigate the correlation of the polymorphisms of the IL1β promoter region 511C/T and tumor necrosis factorα(TNFα) 308G/A gene with the susceptibility of noncardiac gastric cancer of Shaanxi Chinese population. Methods The polymorphisms in the IL1β promoter region and TNFα were determined by PCRRFLP in a Han nationality of Shaanxi Chinese population. Totally 106 cases of noncardiac gastric cancer and 108 cases of nonulcer dyspepsia as controls were subjected to IL1β and TNFα gene analysis. Results IL1β genotype frequencies , CT and TT were %, % and 33% in gastric cancer group; %, %, % in control group respectively. Allele frequencies of C and T were %, % in gastric cancer group; % and % in control group respectively. TNFα308 genotype frequencies of GG, GA and AA were %, % and % in gastric cancer group; %, %, % in control group respectively. Allele frequencies of G and A were % and % in gastric cancer group; % and % in cont
rol group respectively. There were no significant differences in the allele frequency and genotype of IL1β511C/T and TNFα308 (). Conclusion Neither the 511C/T polymorphism in the IL1βpromoter region nor TNFα308 gene polymorp