人参皂苷Rg1对脑挫伤后大鼠脑内胰岛素样生长因子1表达的影响.doc

人参皂苷Rg1对脑挫伤后大鼠脑内胰岛素样生长因子1表达的影响
【摘要】探讨人参皂苷Rg1对脑组织挫伤后大鼠脑中胰岛素样生长因子1(insulinlike growth factor1, IGF1)表达的影响。方法:Wistar大鼠26只,分为正常对照组(2只),脑挫伤模型组(8只)及人参皂苷Rg1治疗组(16只),在损伤后不同时间点(损伤后6 h、12 h、2 d及6 d)分别处死大鼠,使用免疫组化方法观察并检测脑中IGF1的表达。结果:与对照组相比,脑挫伤模型组损伤后各时间点脑组织中IGF1的表达有所升高(P<);与模型组比较,人参皂苷Rg1治疗组IGF1的表达明显升高(P<)。结论:人参皂苷Rg1可能通过促进脑挫伤后脑组织IGF1的表达,促进脑组织的功能恢复。
【关键词】脑挫伤胰岛素样生长因子1 人参皂苷Rg1 大鼠
Objective: To study the effects of ginsenoside Rg1 on the expression of insulinlike growth factor1 (IGF1) in the brain of rats after the experimental brain contusion.
Methods: A total of twentysix Wistar rats were randomly divided into normal control group (n=2), untreated group (n=8) and ginsenoside Rg1 group (n=16). Brain injuries were
induced in rats by a mechanical striking device. The brain tissues were extracted at different times after brain injury (6th hour, 12th hour, 2nd day, 6th day), then the expression of IGF1 in brain tissue was examined by immunohistochemical method.
Results: parison with the normal control group, the expression of IGF1 in the brain tissues was increased in the untreated group after the brain contusion (P<). The expression of IGF1 in brain tissues in ginsenoside Rg1 group was significantly increased pared with the untreated group (P<).
Conclusion: Ginsenoside Rg1 enhances the recovery of the contused brain through increasing the expression of IGF1.
Keywords: brain contusion; insulinlike growth factor1; ginsenoside Rg1; rats
人参皂苷Rg1是人参中提取的一种有效成分,具有神经保护和促进神经再生的作用,近年来有研究证实人参皂苷Rg1的这些作用是通过多种脑内因子实现的[13]。胰岛素样生长因子1(insulinlike growth factor
1, IGF1)是脑损伤后一种重要的内生性神经营养因子,近年来有研究发现创伤性脑损伤及脊髓损伤后,IGF1在神经组织中表达增强[4],可能与脑损伤后神经修复和周围神经损伤后变性有关[5]。本实验采用Feeney's法制作大鼠脑挫伤模型[6],对大鼠注射人参皂苷Rg1,观察其对脑内IGF1表达的影响。
1 材料与方法
实验材料成年Wistar大鼠26只,四川大学华西实验动物中心提供,性别不限,体质量200~250 g。随机分为正常对照组(n=2),模型组(n=8),人参皂苷Rg1组(n=16)。人参皂苷Rg1粉剂,昆明同德众源生物科技有限公司提供,批号110704200605,用生理盐水稀释至10 mg/ml。兔抗大鼠IGF1单克隆抗体(稀释度1︰200)、SP免疫组化染色试剂盒,北京中杉金桥生物技术有限公司提供。二氨基联苯胺显色试剂