多功能Au-Fe3O4纳米颗粒对癌细胞的多模式和超灵敏检测.doc


多功能 Au-Fe3O4 纳米颗粒对癌细胞的多模
式和超灵敏检测#
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(1. 兰州大学应用有机化学国家重点实验室,兰州 730000;
2. 兰州大学第一临床医学院,兰州 730000)
摘要:癌症早期诊断和治疗至关重要,与传统单模式纳米材料相比,多功能纳米复合材料能为癌症早期诊
断和治疗提供更精确的多方位信息。基于 Au-Fe3O4 纳米颗粒的新奇结构,本工作报道了一种偶联叶酸和
铕配合物的 Au-Fe3O4 多功能纳米探针。该纳米探针能应用于叶酸受体过度表达癌细胞(如,HeLa 细胞)
的比色/荧光双模式检测和量化癌细胞。该纳米探针对癌细胞的检测限达 100 个,在生物医学研究中能为
癌症早期诊断提供更准确的信息。
关键词:癌症早期诊断;Au-Fe3O4 纳米颗粒;叶酸;铕配合物;纳米探针;比色/荧光双模式检测
中图分类号:O6
A multifunctional Au–Fe3O4 nanoparticles for multimodal
and ultrasensitive detection of cancer cells
LIU Jian1, ZHANG Wei2, YANG Zhengyin1, LI Tianrong1, HUO Xing1, WANG
Baodui1
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(1. State
Key Laboratory of
Applied
Organic Chemistry, Lanzhou University, Lanzhou,
730000;
2. The First Clinical Medical School, Lanzhou University, Lanzhou, 730000)
Abstract: It is essential for early diagnosis and treatment of cancer cell. Compared with
traditional single-modal nanoparticles, multifunctional nanoparticles can offer more precise and
multiparametric information for the early diagnosis of cancer and better patient management.
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Based on the novel structure of Au-Fe3O4 nanoparticles (NPs), we report folic acid (FA) and
Eu(III) complex-conjugated Au-Fe3O4 NPs for the simultaneous multimodal detection and
counting of cancer cells based on colorimetric/fluorigenic dual-modal detection of cancer cells
with excessive expression folate receptor.