PCR技术及其应用PCR技术在医学中的应用医学分子细胞生物学研究方法WhatisPCRHowtoPCRWhydoPCRDevelopedin1983byKaryMullispolymerasechainreaction(PCR)1993年的诺贝尔化学奖AnimationofPCRPCRReagentsTemplateDNAPrimersdNTPDNAPolymeraseBufferMg2+?InitializationstepDenaturationstepAnnealingstepElongationstepFinalelongationFinalholdPCRprocedure&stages20~40CyclesExponentialamplificationLevelingoffstagePlateauEvolutionofmachinesforPCR1234PCROptimizationContaminationHairpinsPolymeraseerrorsMagnesiumconcentrationNon-specificprimingPrimerdimersDeoxynucleotidesGC-richTemplateProofreadingDNAPolymerasePfu,KOD…DMSO,betaineRedesignPrimersHot-StartPolymerase
PCR技术及其应用 ppt课件 来自淘豆网www.taodocs.com转载请标明出处.