Solid State NMR ... Spectroscopy for Biopolymers - Hazime Sait, Isao Ando, Akira Naito - ( Springer - 2006 - pp.465 ).pdf

SOLID STATE NMR SPECTROSCOPY FOR BIOPOLYMERS
Solid State NMR Spectroscopy
for Biopolymers
Principles and Applications
Hazime Saitoˆ
Himeji Institute of Technology, Himeji, Japan
and Hiroshima University, Hiroshima, Japan
Isao Ando
Tokyo Institute of Technology, Tokyo, Japan
Akira Naito
Yokohama National University, Yokohama, Japan
A . Catalogue record for this book is available from the Library of Congress.
ISBN-10 1-4020-4302-3 (HB)
ISBN-13 978-1-4020-4302-4 (HB)
ISBN-10 1-4020-4303-1 (e-book)
ISBN-13 978-1-4020-4303-1 (e-book)
Published by Springer,
. Box 17, 3300 AA Dordrecht, herlands.
Printer on acid-free paper
All Rights Reserved
ß 2006 Springer
No part of this work may be reproduced, stored in a retrieval system, or transmitted
in any form or by any means, electronic, mechanical, photocopying, microfilming, recording
or otherwise, without written permission from the Publisher, with the exception
of any material supplied specifically for the purpose of being entered
and executed on puter system, for exclusive use by the purchaser of the work.
Printed in herlands.
TABLE OF CONTENTS
Preface ix
Acknowledgements xi
1 Introduction 1
2 Solid State NMR Approach 7
. CP-MAS and DD-MAS NMR 7
. Quadrupolar Nuclei 19
3 Brief Outline of NMR Parameters 31
. Chemical Shift 31
. Relaxation Parameters 42
. Dynamics-Dependent Suppression of Peaks 51
4 Multinuclear Approaches 59
. 31P NMR 59
. 2H NMR 68
. 17O NMR 80
5 Experimental Strategies 89
. Isotope Enrichment (Labeling) 89
. Assignment of Peaks 97
. Ultra-High Field and Ultra-High Speed
MAS NMR 113
6 NMR Constraints for Determination of Secondary Structure 127
. Orientational Constraint 127
. Interatomic Distances 149
. Torsion Angles 173
. Conformation-Dependent Chemical Shifts 181
vi Table of Contents
7 Dynamics 201
. Fast Motions with Motional Frequency>106 Hz 204
. Intermediate or