秦艽地上部分化学成分的分析熊胆粉中牛磺熊去氧胆酸钠和牛磺鹅去氧胆酸钠的分离与含量测定.docx

摘要
(Gentiana macropylla Pall.)、麻花秦艽(Gentiana straminea Maxim.)、粗茎秦艽(Gentiana crassicaulis Duthie ex Burk.) 和小秦艽(Gentiana dahurica Fisch.)的干燥根,是我国重要的传统中药之一,其主要有效成分为龙胆苦苷,具有抗炎、镇痛、保肝、利胆等多种药理作用【30】具有广泛的开发及应用前景。本文从充分利用资源和扩大入药部位出发,对秦艽地上
部分化学成分进行了一定研究运用多种分离方法分离得到6种化合物,并运用波
谱学手段∞V、IR、MS、“C-NMR、1H-NMR、HMBC、HMQC、H—H COSY),
结合理化常数、标准对照品,鉴定了6种化合物的结构。同时采用正交试验法优选出从秦艽地上部分提取龙胆总苷的最佳提取工艺;通过不同型号大孔吸附树脂对龙胆苦苷吸附效果的比较,确定了用于富集龙胆苦苷的大孔吸附树脂的型号: 并通过其对龙胆苦苷的吸附和洗脱试验,确定了最佳工艺条件。此外,采用HPLC 法对秦艽地上部分不同部位龙胆苦苷进行了定量分析;为陕西省教育厅重点科研项目“秦艽地上部分综合开发利用”进行了基础研究。
经胆囊手术引流胆汁而得的干燥品。I临床作为熊胆药物使用,属国家卫生部批准的一类新药,其主要化学成分为结合型胆酸。本文采用经典柱层析法和高效制备液相色谱法相结合从熊胆粉中分离得到牛磺熊去氧胆酸钠和牛磺鹅去氧胆酸钠两种差向异构体,并采用ELSD--HPLC法对熊胆粉中牛磺熊去氧胆酸钠和牛磺鹅去氧胆酸钠进行了定量分析。
关键词: 秦艽地上部分化学成分提取分离结构鉴定龙胆苦苷正交试验大孔吸附树脂}玎?Lc
熊胆粉 牛磺熊去氧胆酸钠 牛磺鹅去氧胆酸钠 高效制备液相
ELSD—HPLC
ABSTRACT
1 Qinjiao is the roots of Gentiana macrophylla Pail.、Gentiana straminea Maxim.、Gentiana crassicaulis Duthie ex Burk and Gentiana dahurica belongs to Genus is an important traditional Chinese ponent gentiopicroside have the effects of anti—inflammatory,stopping pains,
protecting liver and its prospects is order to make full use of the
resource and expand the using parts of Qinjiao,we carried out a systematic research on the chemical constituents in the aerial parts of were achieved by using several separation structures of them were authenticated by the means of spectroscopy(uv、IR、MS、13C-NMR、1H-NMR、HMBC、HMQC The optimum extract method WaS selected by using of orthogonai experiment,and the optimum model of resin was selected paring the adsorptional ability of different resins to by doing adsorption and unadsorption experiments,the best technological conditions was the content of
gentiopicroside in different aerial parts WaS determined by have done
some basic researches for the important project of Shann