实验室分子生物学免役学等常用溶液配.pdf

5. Appendix Contents
General Guidelines for Preparation and Storage
of Buffers and Solutions 89
Commonly Used Buffers and Solutions 90
Spectrophotometric Measurement of Nucleic Acid
Concentration 91
Bacterial Culture Media and Buffers 92
Agarose Gel Electrophoresis Buffers for Analysis of DNA 93
Commonly Used DNA Markers in Agarose Gel
Electrophoresis 93
Southern Transfer Buffers and Solutions 94
Formaldehyde Agarose Gel Electrophoresis Buffers for
Analysis of RNA 94
Northern Transfer Solution 95
Colony-Blot Solutions 95
SDS-PAGE Buffers and Solutions for Analysis of Proteins 96
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5. Appendix
Contents
Continued
Coomassie Staining Solutions 97
Western Transfer Buffers and Solutions 97
Protein Dot-Blot Preparation Buffers 98
Immunodetection Buffers 98
Chromogenic Substrates for Immunoblotting Procedures 99
Coating and Assay Buffers and Solutions for ELISA
Procedures 99
Substrates and Solutions for Protein Assay Procedures 100
QIAGEN Literature 101
QIAGEN on the World Wide Web —
QIAGEN Technical Support 103
Trademarks and Disclaimers 103
This Appendix provides recipes for the media, buffers, and solutions that are used with the protocols described in
the previous chapters. Please be aware that different versions of the same medium, buffer, or solution may exist, so
the recipes described here may differ slightly from those in other molecular biology manuals. The recipes described
here are those used by the scientists at QIAGEN, and provide optimal results. This Appendix also contains
additional useful information for analysis of nucleic acids and proteins, as well as information about different
technical resources offered by QIAGEN.
General Guidelines for Preparation and Storage of Buffers and Solutions
Preparing buffers and solutions
The following guidelines should be followed when preparing buffers and solutions for use in the procedures
described in this Bench Guide.
1. It