医学检验专业本科毕业论文.doc

目录中文摘要………………………………………………………………3英文摘要………………………………………………………………4前言……………………………………………………………………5材料方法………………………………………………………………6结果……………………………………………………………………7讨论……………………………………………………………………7参考文献………………………………………………………………10综述……………………………………………………………………11致谢……………………………………………………………………14三种肺炎支原体检测法的临床应用分析【摘要】目的探讨肺炎支原体(MP)咽拭子快速液体培养法、咽拭子聚合酶链反应(PCR)法和血清MP被动凝集法(MP-Ab)等方法在儿童MP感染诊断治疗过程中的敏感性方法采用3MP检测法对362例临床拟诊呼吸道(非细菌性)感染的患儿的咽拭子和血清标本进行配对研究,每患儿取咽拭子做快速培养和PCR,同时取血清做MP-Ab检测,对3种方法的检测结果与临床诊断治疗病例进行回顾性分析。结果回顾临床病例诊断MP感染患儿152例。MP快速培养法检出阳性78例,%,病程为(±)天;PCR法检测出阳性103例,%,病程为(±)天;MP-Ab法检测出阳性127例,%,病程(±)天。结论3种MP检测法的敏感性与病程有相关性,临床医生应根据患儿病程选取检测方法,以提高阳性检出率和敏感性。【关键词】肺炎支原体,快速培养法,咽拭子聚合酶链反应,血清抗体,配对研究Analysisoftheclinicalapplicationofthreekindsofmycoplasmadetectionmethod【ABSTRACT】ObjectivetoinvestigateMycoplasmapneumoniae(MP)pharynxswabrapidliquidculturemethod,throatswabpolymerasechainreaction(PCR)methodandserumMPpassiveagglutinationassay(MP-Ab)methodinthediagnosisandthetherapyforMPinfectioninchildrenwithsensitivityof362casesclinicallysuspectedrespiratorytractdetectedby3MPmethod(nonbacterial)infectioninchildren'sthroatswabandserumspecimenswerepairedstudy,everychildrenfromthroatswabdofastcultureandPCR,atthesametimeserumMP-Abdetection,,%,courseofdiseasesis(+)days;PCRmethodtodetect103caseswerepositive,%,courseofdiseasesis(+)days;MP-Abmethodtodetectpositivein127cases,%,thecourseofthedisease(+),cliniciansshouldbebasedonchildrenwithdiseasedetectionmethodisselected,inordertoimprovethepositiverateandsensitivity.【keywords】MycoplasmapneumoniaequickcultivationPharyngealswabpolymerasechainreactionserum antibodyPaireddesign前言肺炎支原体(myco