微藻 养殖 培养基.docx

()2XErdschreiber'sMedium   ForUTEXLB2538Directions Erdschreiber' (60ppt). .  #ComponentAmountStockSolution ConcentrationFinalConcentration1SupplementedSeawater3L  2P-IVMetalSolution36mL/3L  3NaNO3 (FisherBP360-500)10mL/·7H2O(SigmaS-9390)10mL/:GR+Medium150mL/3L  6VitaminB123mL/3L1)SupplementedSeawater   Directions For1LTotal ,ponentsintheorderspecifiedwhilestirringcontinuously. .  #ComponentAmountStockSolution ConcentrationFinalConcentration1PasteurizedSeawater900mL  2NaCl(FisherS271-500)23g/L ·6H2O(FisherM33)5g/L  (SigmaS6264)4g/L )PasteurizedSeawater   Directions Mostsalt-watermediausedbyUTEXincludesnaturalseawatercollectedoff-shorefromPortAransas,-filtered,-literbatchofseawaterat30pptina4-literErlenmeyerflaskiscoveredwithasmallinvertedglasspetriplateandaninverted250-mlbeaker,then"pasteurized"-pasteurizedfor45min.,,theinverted-petri-: Invariouslaboratoriesmarinealgaeareculturedinmediapreparedfrom"pasteurized"