微藻-养殖-培养基.docx

() 2X Erdschreiber's Medium   For UTEX LB 2538
Directions 
Erdschreiber's Medium made with Supplemented Seawater instead of Pasteurized Seawater.
For 3 L Total 
1. To 3 L of pasteurized supplemented seawater (60 ppt) aseptically add each of the sterile components in the order specified.
2. Vigorously swirl the contents of the flask to mix thoroughly. 
3. Store at refrigerator temperature.
  
#
Component
Amount
Stock Solution 
Concentration
Final Concentration
1
Supplemented Seawater
3 L
 
 
2
P-IV Metal Solution
36 mL/3 L
 
 
3
NaNO3 (Fisher BP360-500)
10 mL/3 L
M
mM
4
Na2HPO4·7H2O (Sigma S-9390)
10 mL/3 L
M
mM
5
Soilwater: GR+ Medium
150 mL/3 L
 
 
6
Vitamin B12
3 mL/3 L
1)Supplemented Seawater   
Directions 
For 1 L Total 
approximately 900 mL of Pasteurized Seawater, add the following components in the order specified while stirring continuously. 
2. Bring total volume to 1 L with Pasteurized Seawater.
  
#
Component
Amount
Stock Solution 
Concentration
Final Concentration
1
Pasteurized Seawater
900 mL
 
 
2
NaCl (Fisher S271-500)
23 g/L
 
mM
3
MgCl2·6H2O (Fisher M 33)
5 g/L
 
mM
4
Na2SO4 (Sigma S 6264)
4 g/L
 
mM
a）Pasteurized Seawater   
Directions 
Most salt-water media used by UTEX includes natural seawater collected off-shore from Port Aransas, Texas in the Gulf of Mexico. Seawater having a salinity of at least 30 ppt is collected and pre-filtered, then stored undisturbed within polyethylene carboys at ambient temperature.
Seawater is diluted to 30 ppt with double distilled water immediately prior to pasteurization. A three-liter batch of seawater at 30 ppt in a 4-liter Erlenmeyer flask is covered with a small inverted glass petri plate and an inverted 250-ml beaker, then "pasteurized" in a steamer for 45 minutes. The pasteurized content of the flask is allowed to cool and left undisturbed at ambient temperature for appro