应用MTT法进行体外抗肿瘤药敏试验影响因素研究.pdf

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华北国防医药 2008年 12月第 20卷第 6期 Medical Journal of National Defending Forces in North China, Dec. 2008, Vol. 20, No. 6
1 药学与临床
· 临床药学·
应用 MTT法进行体外抗肿瘤药敏试验影响因素研究
魏文青,赵满仓,刘晶,焦娟,张艳,付瑶,安平
(北京军区总医院中心实验科,北京 100700)
[摘要] 目的:探讨应用 MTT法进行体外抗肿瘤药敏试验的影响因素,旨在建立一种 MTT法抗肿瘤药敏试验的规范化流程。方
法:MTT法测定不同浓度人外周血单个核细胞( PBMC)对不同浓度的奥沙利铂与紫杉醇的敏感性,同时优化选择培养时间与 MTT
孵育时间。结果:在每孔 PBMC为 4. 0 × 104 (浓度为 4. 0 × 105 /mL) 、药物作用时间 48 h、MTT 20μL 作用 6 h、单波长 570 nm 处测
OD值时,药物对细胞的抑制率较高,试验效果较好。结论:规范化的 MTT法体外抗肿瘤药敏试验为临床医生开展和实现个性化化
疗提供了可靠的依据。
[关键词] 药物筛选试验,抗肿瘤;体外;MTT法;影响因素
[中图分类号] R979. 1 [文献标识码] A [文章编号] 1009 0878 (2008) 06 0025 03
Influenc ing Factors of the M TT Ba sed on Tum or Chem osen sitiv ity A ssay in V itro( A Study Report)
W E IW en qing, ZHAO Man cang, L IU J ing, J IAO Juan, ZHANG Yan, FU Yao, AN Ping(Department of Central Laboratory, General Hosp ital
of BeijingM ilitary A mand, Beijing 100700, China)
[ Abstract] O bjective: To establish a standard p rocess of measuring the anti cancer drug sensitivity with MTT assay by analyzing the influ
encing factors of the chemosensitivity assay in vitro. M ethods: Some influencing factors ofMTT assay were found in studying the sensitivity of
human peripheral blood mononuclear cells ( PBMC) to anti cancer drugs Oxalip latin and Paclitaxel and the time of selective culture andMTT
incubation time were op tim ized.