《3,5-二硝基水杨酸比色法快速测定烟草水溶性总糖、还原糖及淀粉的探讨》.pdf.pdf

第22卷第6期云南农业大学学报 No 6 2007年11月 Journal ofYunnan A舒cultural University 3,5一二硝基水杨酸比色法快速测定烟草水溶性总糖、还原糖及淀粉的探讨尹建雄1’2,卢红“,谢强2,丁金玲2,李尼杭2 (;,云南昆明650201) 摘要:通过还原糖对3,5一二硝基水杨酸的还原产物的吸收光谱分析,结果表明,该还原产物在500 nm波长下有最大吸收峰,但最佳的工作渡&确定为540 nm。同时找出了显色反应时间及显色剂的最佳用量。井对烟草中水溶性总糖的提取方法进行了优化,建立了批量连续测定烟样中还原糖、水溶性总糖(进行酸水解)、淀粉的快速方法。同时对多个烟样进行丁测定,与伯川法测定结果比较,其相对误差在5%以内。关键词;烟草;比色法;3,5一二硝基水杨酸中图分类号:巧411 文献标识码:A 文章编号:1004—390x(2007)06—0829—05 A Study on Rapid Colorimetric Determination ofWater Soluble Total Sugar,Reducing Sugar and Starch in o with 3,5-dinitrosalicylic Acid YIN dian-xion91,LU Hon92,XlE Qian92,DING din—lin92,LJ Ni—han92 ( o Threshing LID,cO Stone Forest 652200,China; 2 Faculty Tobac∞Scienee,Yurman Agncultural Umvemi哼,Kunming 650201,China) Abstract:肿1e absorption spectrum -dinitrosalicylic acidreducing product with reducing sugar hasheen result shows a maximum absorption at500 thebestwork wavelength was determined by 540 besttime ofcolor reaction andbestsuitable amount ofthe colorreagent was extraction methods of water soluble totalsugar was arapid continuous batch determination ofreducing soluble totalsugar and starch wa8 80mcd o sarnples wei-e telative elTOl'5 oftheresultsobrained by thismethed related tho驼obtained by theBertrand’s method