水中酚及酯类环境雌激素的液相色谱分析.pdf

河北大学
硕士学位论文
水中酚及酯类环境雌激素的液相色谱分析
姓名:彭静
申请学位级别:硕士
专业:分析化学
指导教师:周建科
2011-05
摘要
摘要
水是人类赖以生存的基础,近年来环境雌激素对水体污染的报道日益增多,导致饮
用水安全问题受到广泛关注,加强对饮用水水质的监测非常重要。本文共分三章:第一
章简要介绍了环境雌激素对人体及动物的危害,综述了国内外水中酚类和邻苯二甲酸酯
类(PAEs)物质的分析检测方法,概述了该类物质分析时常见的样品预处理技术。第二
章,采用盐析分相微萃取技术处理水样,用高效液相色谱法检测水中的萘酚、双酚 A 和
氯酚等环境雌激素。选用正丁醇作萃取剂,硫酸铵为盐析效应添加剂,盐酸溶液调 pH=4。
优化了色谱条件,α-萘酚、β-萘酚和双酚 A 在 - mg/L 范围内线性良好,方法检
出限为 - µg/L,加标回收率在 %-%之间,相对标准偏差(RSD)为
%-%;五氯酚在 - mg/L 范围内线性良好,相关系数 ,方法检出限为
µg/L,加标回收率为 %,相对标准偏差为 %。第三章,采用自制微萃取瓶
富集-液相色谱法测定水中壬基酚和 3 种邻苯二甲酸酯类物质。在检测壬基酚时,异辛
烷作萃取剂,盐酸溶液调 pH=,优化了色谱条件,在 -54 mg/L 范围内线性良好,
相关系数 ,方法检出限为 µg/L,加标回收率为 %,相对标准偏差为
%;在检测 PAEs 时,甲苯作萃取剂,邻苯二甲酸二甲酯(DMP)、邻苯二甲酸二乙
酯(DEP)和邻苯二甲酸二丁酯(DBP)在 - mg/L 范围内线性良好,方法检出
限为 - µg/L,加标回收率在 %-%之间,相对标准偏差为 %-%。

关键词水环境雌激素酚类邻苯二甲酸酯类高效液相色谱
I
Abstract
Abstract
Water is the base on which men rely for existence. In recent years, there have been more
and more reports on water pollution caused by environmental estrogens. These reports have
aroused the widespread attention in the society to the safety of drinking water, so it is very
important to enhance the monitoring of water quality. The paper posed of three chapters.
In chapter 1, we introduced the hazard of environmental estrogens to human health and
animals in brief, reviewed analytic detection methods about phenols and phthalate esters
(PAEs) in water at home and abroad, and summarized mon sample pretreatment
methods. In chapter 2, a salting-out phase-separation microextraction /liquid chromatographic
method was used for the determination of environmental estrogens such as naphthol,
bisphenol A and chlorophenol in water. N-butyl alcohol was used as extractant, ammonium
sulfate was used for salting-out effect and pH was 4. The chromatographic conditions were
optimized, α-naphthol, β-naphthol and bisphenol A had good linearity in a range of -5