微藻养殖培养基介绍.docx

1
(NO・1)2XErdschreiber'sMediumForutexlb2538
Directions
Erdschreiber'sMediummadewithSupplementedSeawaterinsteadofPaste1
(NO・1)2XErdschreiber'sMediumForutexlb2538
Directions
Erdschreiber'sMediummadewithSupplementedSeawaterinsteadofPasteurizedSeawater.
(60ppt).
3L
36mL/3L
NaNO°(Fisher
BP360-500)10mL/
Na2HP(S4•392°10mL/
(SigmaS-9390)
150mL/3L


3mL/3L
6
—SupplementedSeawater
11
Directions
,.
1900mL
NaCI(Fisher
2S271-500)
23g/L

MgCl26H2O(FisherM33)
5g/L

Na2SO4(SigmaS
6264)
4g/L

a)PasteurizedSeawater
Directions
Mostsalt-watermediausedbyUTEXincludesnaturalseawatercollectedoff-shorefromPortAransas,-filtered,thenstoredundisturbedwithin
11
polyethylenecarboysatambienttemperature.
-literbatchofseawaterat30pptina4-literErlenmeyerflaskiscoveredwithasmallinvertedglasspetriplateandaninverted250-mlbeaker,then"pasteurized"-pasteurizedfor45min.,,theinverted-petri-.
Procedure:
Invariouslaboratoriesmarinealgaeareculturedinmediapreparedfrom"pasteurized"seawaterthatisassumedtobeheatedtoexactly73degre